Skip to main content

What are Types of Chromatography , Paper chromatography

Types of chromatography

This technique is mostly used for the separation of amino acids, sugars, sugars derivatives and also for the separation of chemicals by this type of chromatography. 

Stationary phase in paper chromatography is Whatman No. 1 or Whatman No. 3 filter papers while Mobile phase is the mixture of water, organic solvents and of various additives.

In paper chromatography mixture of compounds to be separate is allowed to rise up and apply on the upward position (called ascending chromatography) at end of filter paper  (usually 2 cm above the paper) by a capillary action or move down by a capillary action on the paper (called descending chromatography).

Solution of mixture to be separated applied on filter paper and migration of molecules present in solution occur and after some time paper is dried and dipped in solvent to visualize spots on paper with specific coloring reagents. 

Since the speed of migrating molecules is depends on their relative solubility in the organic solvents, the total distance

covered by individual spot relative to the distance moved by the solvent is calculated and is known as Rf(Relative flow).

Sometimes, it is not possible and challenging to separate a complex mixture of substances by a single run with one solvent system. Like such a case, a second run is implemented  by an other

solvent system, in a direction perpendicular to the first run. This is known as two dimensional

chromatography which increase the

separation of a mixture into the

individual components.

Comments

Post a Comment

Popular posts from this blog

What is Meaning of Usman Name

Meaning of Usman name is as  "Usman" (عثمان) name belong to Arabic origin, and it has a great history in islam. This name is very popular in islamic families. Every family at least has a Usman name. Usman name is very common and unique as well. Meaning The name "Usman" means is "strong & powerful" or "wise & intelligent". It is taken from the Arabic word "usman", which signifies strength, courage, and wisdom. Islamic Significance Of Usman Name  In Islamic history, "Usman" is also the name of the third caliph, Uthman ibn Affan (RA), who was known for his wisdom, justice, and piety. Variations You might also come across variations of the name, such as: - Uthman - Usman - Osman (Turkish variant) - Othman Overall, "Usman" is a strong and meaningful name with a significant history and cultural importance!
Post a Comment
Read more

Diabetes and it's types | Diabetes Mellitus

 Introduction to Diabetes  Diabetes is generally defined as “any disorder characterized by excessive urine excretion” There are two types of Diabetes  1) Diabetes Mellitus   2) Diabetes insipidus What is Diabetes Mellitus   Diabetes mellitus is a metabolic disorder of glucose,it is a absolute or relative deficiency of insulin which results in hyperglycemia (increase blood glucose level).  Diabetes mellitus is a common clinical condition and third major cause of death in many developed countries, about 1/5th of individual's above the age of 50, Bearing this disease.   Deficiency of Insulin leads to increased blood glucose level. Thus, due to this high blood glucose, the entry of glucose into the cells is inefficient. Hence, all body cells are starved of glucose. Insulin Dependant Diabetes Mellitus(DDM)  It is also known as Juvenile-onset or Type-I Diabetes Mellitus. There is absolute deficiency of insulin due to viral or auto-immune destr...
Post a Comment
Read more

Difference between RNA and DNA

Easy and short Difference between RNA and DNA is Here   RNA (Ribonucleic Acid) • RNA is a single stranded structure. It is Present mainly in cytoplasm but some in the nucleus. RNA Contains purines (Adenine and Guanine) And Pyrimidines (Cytosine and Uracil). It Contains Ribose(Sugar) and phosphoric acid. DNA (Deoxyribonucleic Acid) DNA is a double stranded helical structure. It is Present mainly in the nucleus but some in the cytoplasm. it is Contains purines (Adenine and Guanine). And pyrimidines (Cytosine and Thymine). It Contains DeoxyRibose (Sugar) and phosphoric acid.
Post a Comment
Read more