Adsorption Column Chromatography

Adsorption column chromatography :

In this technique adsorbents such as silica gel, charcoal powder, alumina and calcium hydroxyapatite are packed into a column in a glass tube (it is serves as the stationary phase).

The sample containing a mixture of components to be separated in a solvent are applied on this column. The components individually get adsorbed on to the adsorbent and then elution is carried out by a buffer

system (mobile phase). The Compounds come one by one out of the column at different speed, which may be separately collected and analyzed. For example amino acids can be determined by ninhydrin colorimetric method.

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