Gel Filtration Chromatography

Gel filtration chromatography : In this technique of chromatography, separation of molecules is accomplished, on the base of their size, shape and molecular weight. Gel filtration chromatography is also known as molecular sieve or molecular exclusion chromatography.

In this type of chromatography a packed column consists of a sponge like gel beads (usually cross-linked polysaccharides) containing pores.

The pores on the surface of gel allows small molecules to penetrate inside, whereas larger molecules are left outside and eluted with a buffer, hence the name exclusion chromatography.

The gels are commercially available in different types (e.g. Sephadex G-25, G-50, G-250, etc.) depending upon the pore size and hence, suitable for different set of molecules

depending on their molecular weight.

Comments

What is Chromatography and its types

The word ‘chromatography’ is derived from Greek word ‘Chromo’ meaning colour and ‘graphy’ meaning to measure; initially it was used to separate coloured compounds from the mixture. In another words It is an analytical technique used for the separation of closely related compounds from a mixture. These compounds are proteins, peptides, lipids ,amino acids, carbohydrates, vitamins and drugs. Chromatography was discovered by Russian Botanist Tswest in 1903. He used it for separation of plant pigments for the first time. The early methods used for the separation and purification of compounds from mixtures were labourious, and s s low. With passage of time and advancement in the separation techniques over years, the chromatographic technique has become to be known as used for the separation of different compounds from mixture and for their identification. Principle of Chromatography Chromatography is usually consists of two phases first a mobile phase and secondary i...

Difference between RNA and DNA

Easy and short Difference between RNA and DNA is Here RNA (Ribonucleic Acid) • RNA is a single stranded structure. It is Present mainly in cytoplasm but some in the nucleus. RNA Contains purines (Adenine and Guanine) And Pyrimidines (Cytosine and Uracil). It Contains Ribose(Sugar) and phosphoric acid. DNA (Deoxyribonucleic Acid) DNA is a double stranded helical structure. It is Present mainly in the nucleus but some in the cytoplasm. it is Contains purines (Adenine and Guanine). And pyrimidines (Cytosine and Thymine). It Contains DeoxyRibose (Sugar) and phosphoric acid.

What is DNA (Deoxyribonucleic Acid)

DNA(Deoxyribonucleic Acid) is a double stranded (double helix) polynucleotide. DNA consists of nitrogenous bases both, purines (adenine and guanine) and pyrimidines (cytosine and thyminc), sugar (deoxyribose) and phosphoric acid. • Uracil (pyrimidine) is not present in DNA. • In DNA the two strands are joined together through out the whole length of the molecule. DNA strands are complementary to cach other but run in opposite directions.The joining between the two strands Always Occurs between purines and pyrimidines and not between purines themselves or pyrimidines themselves. There is a rule, known as "the pairing rule" according to which adenine always joins thymine through three hydrogen bonds (A=T)) and guanine always join cytosine through three hydrogen bonds(G=C). A purine and a pyrimidine, which joins together to form DNA, is known as complementary pair of bases. Therefore adenine (A) and thymine (T) form one complementary pair and guanine (G) and cytosine (C) form t...

Bio and chemistry

Search This Blog