High performance liquid chromatography
(HPLC)
In general, the chromatographic
techniques are time taking and slow. Therefore, the separation of interest can be improved by using high pressure in the range of 5,000-10,000 psi(pounds per square inch), so this technique is commonly known as high pressure liquid chromatography.
This tecnique requires the use of non-compressible resin materials and strong metal columns. At last the eluants of the column are detected by using methods such as fluorescence and UV absorption.
High liquid chromatography is commonly used for the detection and estimation of
vitamins (e.g. vitamin A, calcitriol, etc.), drugs (e.g. phenytoin, phenobarbitones, LSD, AZT, etc.) hormones (e.g. epinephrine, norepinephrine, ACTH, etc.), and metabolites (e.g. metanephrines) in clinical laboratories
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