Skip to main content

High Performance Liquid Chromatography

 High performance liquid chromatography

(HPLC)  

In general, the chromatographic

techniques are time taking and slow. Therefore, the separation of interest can be improved by using high pressure in the range of 5,000-10,000 psi(pounds per square inch), so this technique is commonly known as high pressure liquid chromatography. 

This tecnique requires the use of non-compressible resin materials and strong metal columns. At last the eluants of the column are detected by using methods such as fluorescence and UV absorption.

High liquid chromatography is commonly used for the detection and estimation of

vitamins (e.g. vitamin A, calcitriol, etc.), drugs (e.g. phenytoin, phenobarbitones, LSD, AZT, etc.) hormones (e.g. epinephrine, norepinephrine, ACTH, etc.), and metabolites (e.g. metanephrines) in clinical laboratories


Comments

Post a Comment

Popular posts from this blog

What is Meaning of Usman Name

Meaning of Usman name is as  "Usman" (عثمان) name belong to Arabic origin, and it has a great history in islam. This name is very popular in islamic families. Every family at least has a Usman name. Usman name is very common and unique as well. Meaning The name "Usman" means is "strong & powerful" or "wise & intelligent". It is taken from the Arabic word "usman", which signifies strength, courage, and wisdom. Islamic Significance Of Usman Name  In Islamic history, "Usman" is also the name of the third caliph, Uthman ibn Affan (RA), who was known for his wisdom, justice, and piety. Variations You might also come across variations of the name, such as: - Uthman - Usman - Osman (Turkish variant) - Othman Overall, "Usman" is a strong and meaningful name with a significant history and cultural importance!
Post a Comment
Read more

Diabetes and it's types | Diabetes Mellitus

 Introduction to Diabetes  Diabetes is generally defined as “any disorder characterized by excessive urine excretion” There are two types of Diabetes  1) Diabetes Mellitus   2) Diabetes insipidus What is Diabetes Mellitus   Diabetes mellitus is a metabolic disorder of glucose,it is a absolute or relative deficiency of insulin which results in hyperglycemia (increase blood glucose level).  Diabetes mellitus is a common clinical condition and third major cause of death in many developed countries, about 1/5th of individual's above the age of 50, Bearing this disease.   Deficiency of Insulin leads to increased blood glucose level. Thus, due to this high blood glucose, the entry of glucose into the cells is inefficient. Hence, all body cells are starved of glucose. Insulin Dependant Diabetes Mellitus(DDM)  It is also known as Juvenile-onset or Type-I Diabetes Mellitus. There is absolute deficiency of insulin due to viral or auto-immune destr...
Post a Comment
Read more

Difference between RNA and DNA

Easy and short Difference between RNA and DNA is Here   RNA (Ribonucleic Acid) • RNA is a single stranded structure. It is Present mainly in cytoplasm but some in the nucleus. RNA Contains purines (Adenine and Guanine) And Pyrimidines (Cytosine and Uracil). It Contains Ribose(Sugar) and phosphoric acid. DNA (Deoxyribonucleic Acid) DNA is a double stranded helical structure. It is Present mainly in the nucleus but some in the cytoplasm. it is Contains purines (Adenine and Guanine). And pyrimidines (Cytosine and Thymine). It Contains DeoxyRibose (Sugar) and phosphoric acid.
Post a Comment
Read more