Skip to main content

Ion Exchange Chromatography

 Ion-exchange chromatography : Ion exchange chromatography is the process In which separation of molecules is carried out by on the basis of their electrical charges. For this purpose Ion-exchange resins, cation exchangers and anion exchangers are employed. In this method of separation an anion exchanger (R+A) donates its anion (A) with another anion (B) in solution

R+A+ B ⇌R+B+ A

Likewise, a cation exchanger (H+R) donates its cation (H+) with another cation (C+) in solution.

H+R + C⇌ C+R + H

Therefore, ions in solution are reversibly replaced by ion exchange resins in ion exchange chromatography. This depends on their binding abilities of ions

bearing positive or negative charges are highly pH dependent, since the net charge on the surface of molecules varies with

pH. This principle is helpful in the ion-exchange chromatography for the separation of molecules.

ion-exchange chromatography

Conveniently mixture of proteins or amino acids can be separated by ion exchange chromatography.

Comments

Post a Comment

Popular posts from this blog

What is Chromatography and its types

The word ‘chromatography’ is derived from Greek word ‘Chromo’ meaning colour and ‘graphy’ meaning to measure; initially it was used to separate coloured compounds from the mixture.   In another words It is an analytical technique used for the separation of closely related compounds from a mixture. These compounds are proteins, peptides, lipids ,amino acids, carbohydrates, vitamins and drugs. Chromatography was discovered by Russian Botanist Tswest in 1903. He used it for separation of plant pigments for the first time. The early methods used for the separation and purification of compounds from mixtures were labourious, and s  s low.  With passage of time and advancement in the separation techniques over years, the chromatographic technique has become to be known as used for the separation of different compounds from mixture and for their identification. Principle of Chromatography Chromatography is usually  consists of two phases first a mobile phase and secondary i...
Post a Comment
Read more

Difference between RNA and DNA

Easy and short Difference between RNA and DNA is Here   RNA (Ribonucleic Acid) • RNA is a single stranded structure. It is Present mainly in cytoplasm but some in the nucleus. RNA Contains purines (Adenine and Guanine) And Pyrimidines (Cytosine and Uracil). It Contains Ribose(Sugar) and phosphoric acid. DNA (Deoxyribonucleic Acid) DNA is a double stranded helical structure. It is Present mainly in the nucleus but some in the cytoplasm. it is Contains purines (Adenine and Guanine). And pyrimidines (Cytosine and Thymine). It Contains DeoxyRibose (Sugar) and phosphoric acid.
Post a Comment
Read more

What is DNA (Deoxyribonucleic Acid)

 DNA(Deoxyribonucleic Acid) is a double stranded (double helix) polynucleotide. DNA consists of nitrogenous bases both, purines (adenine and guanine) and pyrimidines (cytosine and thyminc), sugar (deoxyribose) and phosphoric acid. • Uracil (pyrimidine) is not present in DNA. • In DNA the two strands are joined together through out the whole length of the molecule. DNA strands are complementary to cach other but run in opposite directions.The joining between the two strands Always Occurs between purines and pyrimidines and not between purines themselves or pyrimidines themselves. There is a rule, known as "the pairing rule" according to which adenine always joins thymine through three hydrogen bonds (A=T)) and guanine always join cytosine through three hydrogen bonds(G=C). A purine and a pyrimidine, which joins together to form DNA, is known as complementary pair of bases. Therefore adenine (A) and thymine (T) form one complementary pair and guanine (G) and cytosine (C) form t...
Post a Comment
Read more