Thin Layer Chromatography

Thin layer chromatography (TLC)

The working principle of thin layer chromatography is similar to paper chromatography. In TLC in place of a paper, an inert substance, such as cellulose, is Placed as supporting material.

Different grades of cellulose are spread on the glass or plastic plates which are then activated at high temperatures.

Comparatively the chromatographic separation carried out in TLC is rapid.

In TLC adsorption, such alumina, kieselguhr, and activated silica gel are used.

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What is Chromatography and its types

The word ‘chromatography’ is derived from Greek word ‘Chromo’ meaning colour and ‘graphy’ meaning to measure; initially it was used to separate coloured compounds from the mixture. In another words It is an analytical technique used for the separation of closely related compounds from a mixture. These compounds are proteins, peptides, lipids ,amino acids, carbohydrates, vitamins and drugs. Chromatography was discovered by Russian Botanist Tswest in 1903. He used it for separation of plant pigments for the first time. The early methods used for the separation and purification of compounds from mixtures were labourious, and s s low. With passage of time and advancement in the separation techniques over years, the chromatographic technique has become to be known as used for the separation of different compounds from mixture and for their identification. Principle of Chromatography Chromatography is usually consists of two phases first a mobile phase and secondary i...

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